Composite

Part:BBa_K4164011:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)


constitutive expression of FXR-ddRFPA1 and RXR-ddRFPB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2284
    Illegal NheI site found at 2307
    Illegal NheI site found at 4199
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1533
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2689
    Illegal AgeI site found at 3716
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3071
    Illegal SapI.rc site found at 1122


Design Notes

We recombined BBa_K4164009 and BBa_K4164010 by homologous recombination.

Source

This sequence is composed of BBa_K416009 and BBa_K416010.

References